Center for the Chemistry of Cellular Signaling

Dedicated to understanding the intricacies of cellular signaling, the Center for the Chemistry of Cellular Signaling was built on Caltech's successes at the interface of chemistry and biology. It is focused on determining how complex systems of molecules interact to create the pathways that regulate the lives of cells and allow them to respond to their environments. Rather than just studying a complex biomolecule in isolation, an understanding of how it is part of a larger path of molecules is the purpose of its establishment. The Center was made possible by a generous grant from the Gordon and Betty Moore Foundation. 

If use of the Center instrumentation has contributed significantly to your research, we request that you cite the Center in the acknowledgments section of your publication.  For example: "This work was supported by…, and by the Center for the Chemistry of Cellular Signaling at California Institute of Technology."

Instrumentation:

Crellin Room 260 (Bacterial)

Crellin Room 262 (Mammalian)

People in the Facility:

  • Faculty members
    Dr. Dennis Dougherty
    Dr. Jacqueline Barton
    Dr. Peter Dervan
    Dr. Linda Hsieh-Wilson
    Dr. Shu-ou Shan
    Dr. Long Cai 
     
  • Grad Lab Assistants:
    Anna Arnold (Barton group)
    Katie Schaefer (Barton group)
    Alyson Weidmann (Barton group)
    JJ Kang (Dervan group)
    Bryce Jarman (Dougherty group)
    Meera Rao (Shan group)
    Tima Zhiyentayev (Cai group)
    Andrew Wang (Hsieh-Wilson group)

Seminars:

1st and 3rd Thursdays of the month
151 Crellin
12:00 to 1:00 p.m.
Upcoming Seminars:
October 2, 2014
October 16, 2014
November 6, 2014
November 20, 2014
December 4, 2014


 

Instrument Signup:


An online signup calendar is available. 
Username: cellsig and password: cce12345.

 

Information on Instrumentation

For each of the instruments, please contact the indicated GLA for training or troubleshooting. 

Cell Disruptor

The Protein Expression Center (PEC) in the Beckman Institute (room 286) has a new cell disruptor that is free to Signaling Center users.  The cell disruptor is a Constant Systems TS Series Benchtop model.  Compared to a microfluidizer, this instrument can reach higher pressures for yeast lysis (and easily be used at lower pressures for bacteria).  The instrument is equipped with a circulating chiller for cooling to desired temperature, i.e. 4 degrees Celsius.

Contact Sravya to set up training (sravya@caltech.edu).  You will be asked to set up an online account with PEC and then submit an order online for training or cell disruptor use.  On the site, select the "Protein Downstream Processing" and the "Cell lysis" options.  Use of this cell disruptor will be free for Signaling Center users with user fees charged to group-specific Signaling Center PTAs.

 

 

Flexstation 3

Microplate reader with 5 read modes (including absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence) with additional robot functionality (8-channel pipettor) to add liquid reagents.

Contact: Bryce Jarman (Dougherty), x6009, jjarman@caltech.edu

Flexstation 3

Odyssey

Quantitative scanning of western blots at 700 and 800 nm.

Contact: Andrew Wang (Hsieh-Wilson), x8119, acwang@caltech.edug

Odyssey

CFX 96 Real-Time PCR Detection System

Built upon the Bio-Rad C1000 96 well Thermal Cycler, utilizes filtered LEDs for excitation and is capable of monitoring 450-750 nM excitation/emission wavelength range. This system allows for simultaneous detection of up to 5 different fluorophores per reaction and is sensitive enough to detect one copy of target sequence in human genomic DNA.

Contact: Katie Schaefer (Barton), x3201, schaefer@caltech.edu 

CFX 96 Real-Time PCR Detection System

Nanodrop 2000

Micro-volume full spectrum UV-Visible spectrophotometer for sample volumes as low as 0.5 µL.

Contact: Tima Zhiyentayev (Cai), x2617, tima@caltech.edu

Nanodrop 2000

QIAcube

Fully automated sample preparation of Qiagen spin-column kits (up to 12 samples per run) for purification of DNA, RNA or proteins.

Contact: Katie Schaefer (Barton), x3201, schaefer@caltech.edu 

QIAcube

I2500 KC Incubator Shaker

Floor-mounted shaker accepts up to 6-L flasks for culture.

Contact: Meera Rao (Shan), x4071, meera.rao@caltech.edu

I2500 KC Incubator Shaker

Innova 43 Incubator Shaker

Floor-mounted temperature-controlled shaker accepts up to 6-L flasks for culture. Can go down to 5°C.

Contact: Meera Rao (Shan), x4071, meera.rao@caltech.edu

Innova 43 Incubator Shaker

Avanti J-26 Centrifuge

Centrifuge up to 6 1-Liter cultures.

Contact: Meera Rao (Shan), x4071, meera.rao@caltech.edu

Avanti J-26 Centrifuge

Ultrospec 10

Portable spectrophotometer for OD600 readings.

Ultrospec 10

-80°C freezer

Intended for use as a back-up of individual lab freezers.

Contact: Tima Zhiyentayev (Cai), x2617, tima@caltech.edu

-80°C freezer

Typhoon FLA 9000

High-resolution biomolecular imager capable of detecting storage phosphor, chemiluminescence, and multiplex fluorescence with 473 nm, 532 nm, and 625 nm excitation. Analysis done with ImageQuant software.

Contact: JJ Kang (Dervan), x6032, jjskang@caltech.edu

Typhoon FLA 9000

Amaxa 4D-Nucleofector

Efficient electroporation of mammalian cells for transfection.

Contact: Andrew Wang (Hsieh-Wilson), x8119, acwang@caltech.edug

Amaxa 4D-Nucleofector

Neon Transfection System

High efficiency electroporation transfection device for primary cells, stem cells, and difficult-to-transfect cells. It is capable of transfecting 10 or 100 µL volumes.

Contact: Bryce Jarman (Dougherty), x6009, jjarman@caltech.edu

Neon Transfection System

Nikon SMZ1000 Microscope

Used for dissections and imaging (transmitted light) with the camera attached. Zoom from 0.8 to 8X, in a clean bench to provide a sterile dissection/preparation work space.

Contact: Tima Zhiyentayev (Cai), x2617, tima@caltech.edu

Nikon SMZ1000 Microscope

Nikon TS100 Microscope

Used for basic visualization of cultured cells at 10 and 20X, allowing for monitoring and counting cells.

Contact: Tima Zhiyentayev (Cai), x2617, tima@caltech.edu

Nikon TS100 Microscope

Clean Bench

Provides sterile environment for sensitive dissections or microscope work.

Contact: Andrew Wang (Hsieh-Wilson), x8119, acwang@caltech.edug

Clean Bench

Laminar Flow Hoods

Provides sterile environment for mammalian cell work.

Contact: Alyson Weidmann (Barton), x3201, aweidman@caltech.edu

Laminar Flow Hoods

Mammalian Tissue Culture COIncubator

Provides a controlled CO2 and temperature environment for cells during dissection, transfection or passaging done at the Center. It is intended for temporary use, and not long term housing of cultured cells.

Contact: Alyson Weidmann (Barton), x3201, aweidman@caltech.edu

 

Eppendorf Centrifuge 5702

Tabletop centrifuge that can accomodate 15 or 50 mL conical tubes.

Contact: JJ Kang (Dervan), x6032, jjskang@caltech.edu

Eppendorf Centrifuge 5702

Mailing address

Caltech Center for the Chemistry of Cellular Signaling
Division of Chemistry and Chemical Engineering
MC 164-30
California Institute of Technology
Pasadena, CA 91125
USA