| Center for the Chemistry of Cellular Signaling Dedicated to understanding the intricacies of cellular signaling, the Center for the Chemistry of Cellular Signaling was built on Caltech's successes at the interface of chemistry and biology. It is focused on determining how complex systems of molecules interact to create the pathways that regulate the lives of cells and allow them to respond to their environments. Rather than just studying a complex biomolecule in isolation, an understanding of how it is part of a larger path of molecules is the purpose of its establishment. The Center was made possible by a generous grant from the Gordon and Betty Moore Foundation. Instrumentation
Crellin Room 260 (Bacterial)
Crellin Room 262 (Mammalian)
Please see these links for details on: |
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For each of the instruments, please contact the indicated GLA for training or troubleshooting.
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Microplate reader with 5 read modes (including absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence) with additional robot functionality (8-channel pipettor) to add liquid reagents. Contact: Kristina |
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Quantitative scanning of western blots at 700 and 800 nm. Contact: Andrew |
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CFX 96 Real-Time PCR Detection System CFX 96 Real-Time PCR Detection System (Katie) Built upon the Bio-Rad C1000 96 well Thermal Cycler, utilizes filtered LEDs for excitation and is capable of monitoring 450-750 nM excitation/emission wavelength range. This system allows for simultaneous detection of up to 5 different fluorophores per reaction and is sensitive enough to detect one copy of target sequence in human genomic DNA. Contact: Katie |
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Micro-volume full spectrum UV-Visible spectrophotometer for sample volumes as low as 0.5 µL. Contact: Tima |
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Fully automated sample preparation of Qiagen spin-column kits (up to 12 samples per run) for purification of DNA, RNA or proteins. Contact: Katie |
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Floor-mounted shaker accepts up to 6-L flasks for culture. Contact: Thang |
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Floor-mounted temperature-controlled shaker accepts up to 6-L flasks for culture. Can go down to 5°C. Contact: Thang |
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Centrifuge up to 6 1-Liter cultures. Contact: Thang |
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Portable spectrophotometer for OD600 readings. |
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Intended for use as a back-up of individual lab freezers. Contact: Tima |
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High-resolution biomolecular imager capable of detecting storage phosphor, chemiluminescence, and multiplex fluorescence with 473 nm, 532 nm, and 625 nm excitation. Analysis done with ImageQuant software. Contact: JJ |
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Efficient electroporation of mammalian cells for transfection. Contact: Andrew |
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High efficiency electroporation transfection device for primary cells, stem cells, and difficult-to-transfect cells. It is capable of transfecting 10 or 100 µL volumes. Contact: Kristina |
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Used for dissections and imaging (transmitted light) with the camera attached. Zoom from 0.8 to 8X, in a clean bench to provide a sterile dissection/preparation work space. Contact: Tima |
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Used for basic visualization of cultured cells at 10 and 20X, allowing for monitoring and counting cells. Contact: Tima |
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Provides sterile environment for sensitive dissections or microscope work. Contact: Andrew |
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Provides sterile environment for mammalian cell work. Contact: Alyson |
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Mammalian Tissue Culture CO2 Incubator Provides a controlled CO2 and temperature environment for cells during dissection, transfection or passaging done at the Center. It is intended for temporary use, and not long term housing of cultured cells. Contact: Alyson |
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Tabletop centrifuge that can accomodate 15 or 50 mL conical tubes. Contact: JJ |
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California Institute of Technology. All Rights Reserved. Webmaster: chemwww caltech.edu. |
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