Mass
Spectrometry Facility instrumentation consists of:
Link here for details on: Operation, Schedule, Fees, Contact Information, and Useful Links. Mass
Spectrometry Request Form (pdf)
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Hewlett-Packard 5970B MSD with 5890 GC for GC-MS analysis This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass limit 500 Da). The gas chromatograph uses a DB-5 capillary column that is useful for separation of most non-polar species. |
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Finnigan LCQ ion trap mass spectrometer with Surveyor Autosampler and HPLC pump The LCQ is equipped with an electrospray ionization source, and is suited for mass analysis of a broad range of molecules that are relatively polar, nonvolatile and thermally labile. The tendency to form multiply charged species during ionization extends the available mass range (normally 2000 Da) to higher limits. The LCQ may be operated in direct infusion mode or with an LC. In direct infusion analysis, the analyte solution is in a syringe and flows to the electrospray source using a syringe pump. In LC-MS, the analyte solution is injected onto an HPLC column and the column eluent flows to the electrospray source. Currently, the LC-MS option is dedicated primarily to microscale analysis of protein digests. |
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Applied Biosystems Voyager DE-PRO MALDI TOF-MS The DE-PRO is a time-of-flight (TOF) mass spectrometer equipped with a matrix-assisted laser desorption ionization (MALDI) ion source. It uses a 20-Hz nitrogen laser and is capable of operation in linear and/or reflected ion modes. The "unlimited" mass range of the TOF mass spectrometer and "soft ionization" characteristic of MALDI enable analysis of many macromolecules including large proteins, oligonucleotides, polysaccharides and select synthetic polymers. Training Video: View Quicktime Movie |
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JEOL JMS-600H High Resolution Mass Spectrometer The JMS-600H is a compact double-focusing, high-resolution, magnetic sector mass spectrometer with resolving power of 25,000, using the 10% valley definition. This instrument will be used for exact mass measurement of molecules. Samples may be introduced into the ion source using a gas chromatograph (GC) or a direct insertion probe (DIP) and ionized by electron ionization (EI) and/or chemical ionization (CI). Polar and nonvolatile samples are usually introduced into the ion source by the FAB probe and ionized by fast atom bombardment (FAB). |
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Operation Infrequent MS users as well as off-campus individuals may submit samples for analysis. Please use Mass Spectrometry Request Form provided. Schedule
(Review This Section Often) Mondays and Tuesdays are reserved primarily for LC-MS operation. The remaining days are reserved for direct infusion analysis. Please check notices on the instrument as this schedule may change with changing projects and needs in the division. The LC-MS operation is primarily focused on microscale analysis of protein digests. Fees Times of use will be rounded off to 1/4 hour or 15 minutes. Service fees for samples submitted for routine mass analysis are $40 for university users and $100 for non-university users. Additional fees will be charged for samples requiring clean-up and extensive preparation.
Mailing address
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California Institute of Technology. All Rights Reserved. Webmaster: chemwww caltech.edu. |
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